Summary
Purpose
The main aim of the
BLIS workshop is to describe the latest theory and practice of low- and
intermediate- energy synchrotron radiation (SR) techniques that have become
popular in biology research. Unlike large expensive high-energy storage
rings designed to produce x-rays (>1 keV) for protein crystallography,
low-energy storage rings such as ASTRID produce radiation optimised for
the study of molecular electronic transitions (1-100 eV) and makes available
a host of photobiology techniques. Topic sessions will cover a variety
of techniques over a wide range of applications at the molecular,
cellular and organism level. The fusion of biological and physical sciences
at the workshop is expected to encourage cross-disciplinary as well as
inter-technique dialogue among biologists, chemists, engineers and physicists.
A vital aspect of the BLIS workshop is the inspiration of young scientists
to pursue research in the field of SR, its related technologies and applications.
To this end we plan to fund some student attendees.
The major part
of the workshop will be dedicated to lectures by experts on SR based techniques
and biological applications. Additionally, afternoon sessions are reserved
for poster and oral presentations by attendees on related topics. There
will also be the opportunity to gain 'hands-on' experience, performing
experiments on their own samples
using the
CD, XM and GBS facilities. Please contact the organisers to discuss specimen
requirements and machine specifications as soon as possible.
Techniques
Circular
Dichroism (CD) spectroscopy: CD takes advantage of the intrinsic chirality
of nature using the differential absorption of left- and right-circularly
polarised light to determine secondary structure content of proteins and
nucleic acids. Protein molecule stability and conformational changes can
be studied as a function of environment as well as folding and unfolding
processes under normal and abnormal physiological conditions. The SR provided
by ASTRID permits new information on molecular structure to be gathered
at lower wavelengths, higher data rates and increased signal-to-noise ratios;
thereby allowing more extensive experiments to be performed, such
as stop-flow. Attendees will be given the opportunity to use SR CD on ASTRID
during the workshop. Contact John Kenney for more information.
Fourier
Transform Infrared (FT IR) Spectroscopy: FT IR is also used to study
the secondary structure of proteins, which can be interpreted from their
characteristic infrared spectra. FT IR data is often used to complement
the UV data of CD. Low energy SR facilities, such as ASTRID, are ideal
sources of tuneable IR to get clean data, especially for applications requiring
high intensity, such as FT IR microscopy.
Fluorescence
Spectroscopy: Fluorescence is used to study biomolecular dynamics on
a very short time scale. It can also be coupled with other techniques,
e.g., CD, to further elucidate molecular structure. Pulsed sources of SR
in the visible, UV and VUV can be used to probe the molecular behaviour
on very short time scales.
X-ray Microscopy
(XM): XM takes advantage of the so-called water window, accessible
using SR, to image thick (up to 10 µm) hydrated biological specimens
with high contrast. High-resolution (20-50 nm) images can be obtained of
whole cells in solution without chemical fixation, staining or drying.
Cells are alive up until the moment the image is taken. Attendees will
be given the opportunity to use the transmission XM on ASTRID during the
workshop. Contact Joanna Abraham-Peskir for more information.
Medical
tomography: Tomography on a microscopic scale (with µm-level
resolution) with is only possible using SR. The technique is particularly
suitable for characterising pathological cell structure in bone.
Gas-phase
Biomolecule Spectroscopy (GBS): The electrostatic storage ring ELISA
can store large biomolecule ions for gas-phase experiments. This unique
facility provides the opportunity to make rapid lifetime studies of biomolecules
to study conformational changes and should provide valuable information
on protein dynamics. Attendees interested in GBS on ELISA and especially
to get experience with the technique during the workshop should contact
Søren Pape Møller for more information. |